Journal: Journal of Inflammation Research

Article Title: Disulfidptosis-Induced Chondrocyte-Macrophage Crosstalk via GYS1/CCND1/NOD2 Axis Promotes Osteoarthritis Progression

doi: 10.2147/JIR.S568766

Figure Lengend Snippet: GYS1-Mediated Disulfidptosis Drives M1 Macrophage Polarization in the Osteoarthritic Niche. ( A ) Volcano plot of DEGs in GSE114007 re-stratified by four disulfidptosis-associated genes. ( B ) Venn diagram intersecting DEGs from four subgroups with M1 macrophage-associated genes, yielding 23 overlapping DEGs. ( C ) Intersection of 23 DEGs with M1 macrophage hub genes identifies 3 core DEGs. ( D ) Expression patterns of core DEGs across re-stratified subgroups. ( E ) Immunohistochemical staining of GYS1 protein. The higher-magnification images with black and green borders on the right correspond to the areas enclosed by the black and green rectangles in the lower-magnification image on the left. ( F ) Phalloidin staining of ATDC5 cells. ( G ) RT-qPCR assay for disulfidptosis-related gene expression in ATDC5 cells. ( H ) RT-qPCR assay for macrophage polarization and target gene expression in the ATDC5 and RAW264.7 co-culture system. ( I ) ROC analysis of GYS1. ( J ) ROC analysis of CCND1 and NOD2. Data were expressed as the mean ± standard deviation (SD). *p < 0.05 vs High group by t -test for panels ( C ). *p < 0.05 vs normal by t -test for panels ( D ). *p < 0.05 vs Glu-, #p < 0.05 vs Glu+ by One-Way ANOVA for panels ( G and H ).

Article Snippet: The ATDC5 (mouse chondrogenic cell line) and RAW264.7 (mouse monocytic macrophage cell line) were obtained from the European Collection of Authenticated Cell Cultures (ECACC) and the American Type Culture Collection (ATCC), respectively, and maintained in DMEM (Gibco, USA) supplemented with 10% FBS (Wisent, Canada) at 37 °C with 5% CO 2 .

Techniques: Expressing, Immunohistochemical staining, Staining, Quantitative RT-PCR, Gene Expression, Targeted Gene Expression, Co-Culture Assay, Standard Deviation